Fast phase processing in off-axis holography using multiplexing with complex encoding and live-cell fluctuation map calculation in real-time (2024)

Girshovitz, P., & Shaked, N. T. (2015). Fast phase processing in off-axis holography using multiplexing with complex encoding and live-cell fluctuation map calculation in real-time. Optics Express, 23(7), 8773-8787. https://doi.org/10.1364/OE.23.008773

Girshovitz, Pinhas ; Shaked, Natan T. / Fast phase processing in off-axis holography using multiplexing with complex encoding and live-cell fluctuation map calculation in real-time. In: Optics Express. 2015 ; Vol. 23, No. 7. pp. 8773-8787.

@article{b980618bc26942698c96ccfb0a75a33b,

title = "Fast phase processing in off-axis holography using multiplexing with complex encoding and live-cell fluctuation map calculation in real-time",

abstract = "We present efficient algorithms for rapid reconstruction of quantitative phase maps from off-axis digital holograms. The new algorithms are aimed at speeding up the conventional Fourier-based algorithm. By implementing the new algorithms on a standard personal computer, while using only a single-core processing unit, we were able to reconstruct the unwrapped phase maps from one megapixel off-axis holograms at frame rates of up to 45 frames per second (fps). When phase unwrapping is not required, the same algorithms allow frame rates of up to 150 fps for one megapixel off-axis holograms. In addition to obtaining realtime quantitative visualization of the sample, the increased frame rate allows integrating additional calculations as a part of the reconstruction process, providing sample-related information that was not available in real time until now. We use these new capabilities to extract, for the first time to our knowledge, the dynamic fluctuation maps of red blood cells at frame rate of 31 fps for one megapixel holograms.",

author = "Pinhas Girshovitz and Shaked, {Natan T.}",

note = "Publisher Copyright: {\textcopyright} 2015 Optical Society of America.",

year = "2015",

month = apr,

day = "6",

doi = "10.1364/OE.23.008773",

language = "English",

volume = "23",

pages = "8773--8787",

journal = "Optics Express",

issn = "1094-4087",

publisher = "The Optical Society",

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}

Girshovitz, P & Shaked, NT 2015, 'Fast phase processing in off-axis holography using multiplexing with complex encoding and live-cell fluctuation map calculation in real-time', Optics Express, vol. 23, no. 7, pp. 8773-8787. https://doi.org/10.1364/OE.23.008773

Fast phase processing in off-axis holography using multiplexing with complex encoding and live-cell fluctuation map calculation in real-time. / Girshovitz, Pinhas; Shaked, Natan T.
In: Optics Express, Vol. 23, No. 7, 06.04.2015, p. 8773-8787.

Research output: Contribution to journal › Article › peer-review

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AU - Girshovitz, Pinhas

AU - Shaked, Natan T.

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N2 - We present efficient algorithms for rapid reconstruction of quantitative phase maps from off-axis digital holograms. The new algorithms are aimed at speeding up the conventional Fourier-based algorithm. By implementing the new algorithms on a standard personal computer, while using only a single-core processing unit, we were able to reconstruct the unwrapped phase maps from one megapixel off-axis holograms at frame rates of up to 45 frames per second (fps). When phase unwrapping is not required, the same algorithms allow frame rates of up to 150 fps for one megapixel off-axis holograms. In addition to obtaining realtime quantitative visualization of the sample, the increased frame rate allows integrating additional calculations as a part of the reconstruction process, providing sample-related information that was not available in real time until now. We use these new capabilities to extract, for the first time to our knowledge, the dynamic fluctuation maps of red blood cells at frame rate of 31 fps for one megapixel holograms.

AB - We present efficient algorithms for rapid reconstruction of quantitative phase maps from off-axis digital holograms. The new algorithms are aimed at speeding up the conventional Fourier-based algorithm. By implementing the new algorithms on a standard personal computer, while using only a single-core processing unit, we were able to reconstruct the unwrapped phase maps from one megapixel off-axis holograms at frame rates of up to 45 frames per second (fps). When phase unwrapping is not required, the same algorithms allow frame rates of up to 150 fps for one megapixel off-axis holograms. In addition to obtaining realtime quantitative visualization of the sample, the increased frame rate allows integrating additional calculations as a part of the reconstruction process, providing sample-related information that was not available in real time until now. We use these new capabilities to extract, for the first time to our knowledge, the dynamic fluctuation maps of red blood cells at frame rate of 31 fps for one megapixel holograms.

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Girshovitz P, Shaked NT. Fast phase processing in off-axis holography using multiplexing with complex encoding and live-cell fluctuation map calculation in real-time. Optics Express. 2015 Apr 6;23(7):8773-8787. doi: 10.1364/OE.23.008773